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Author:   Ian Stansfield (University of Aberdeen, Foresterhill, U.K.) ,  Mike Stark (University of Dundee, U.K.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   2nd edition
Volume:   v. 36
Dimensions:   Width: 16.80cm , Height: 4.30cm , Length: 24.40cm
Weight:   1.400kg
ISBN:  

9780123694799

ISBN 10:   0123694795
Pages:   730
Publication Date:   09 July 2007
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Unknown
Availability:   Awaiting stock  

Table of Contents

Introduction to functional analysis in yeast Yeast genetics and strain construction Yeast transformation A guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting) Studying essential genes: generating and using promoter fusions and conditional alleles Yeast hybrid approaches Array-based yeast two-hybrid screening for protein-protein interactions Reporter genes and their uses in studying yeast gene expression Transcript analysis: a microarray approach GFP-based microscopic approaches for whole chromosome analysis in yeasts Immunological methods Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins Yeast protein microarrays Smart genetic screens High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae Chemical genomic tools for understanding gene function and drug action RNA gene analysis Analysis of gene function of mitochondria Yeast prions and their analysis in vivo Metabolic control in the eukaryotic cell, a Systems Biology perspective Phylogenetic footprinting Saccharomyces cerevisiae as a tool for human gene function discovery Bioinformatic prediction of yeast gene function Yeast genetic strain and plasmid collections Yeast gene analysis: the future

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