Visualization of Receptors In Situ: Applications of Radioligand Binding

Author:   Emmanuel Moyse ,  Slavica M Krantic ,  Slavica M Krantic (INSERM, Lyon, Cedex, France)
Publisher:   Taylor & Francis Inc
ISBN:  

9780849300424


Pages:   204
Publication Date:   30 November 2000
Format:   Hardback
Availability:   In Print   Availability explained
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Visualization of Receptors In Situ: Applications of Radioligand Binding


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Overview

Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not encountered in the original research papers - helping the reader overcome the challenges associated with this powerful technique.

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Author:   Emmanuel Moyse ,  Slavica M Krantic ,  Slavica M Krantic (INSERM, Lyon, Cedex, France)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Dimensions:   Width: 17.80cm , Height: 1.60cm , Length: 25.40cm
Weight:   0.586kg
ISBN:  

9780849300424


ISBN 10:   0849300428
Pages:   204
Publication Date:   30 November 2000
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.

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Emmanuel Moyse, Slavica M Krantic

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