Overview

"This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.   Chapter 10, ""Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos,"" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com."

Full Product Details

Author:   Francisco J. Pelegri
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2019
Volume:   1920
Weight:   1.056kg
ISBN:  

9781493990085

ISBN 10:   149399008
Pages:   434
Publication Date:   09 February 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments Douglas W. Houston 2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos Thao Nguyen, Timothy J. Mitchison, and Martin Wuhr 3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos Michael D. Sheets 4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts Alexander A. Tokmakov and Ken-Ichi Sato 5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis Ken-ichi Sato and Alexander A. Tokmakov 6. Embryological and Genetic Manipulation of Chick Development Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal 7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos Ana Paula Azambuja and Marcos Simoes-Costa 8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens Triveni Menon and Sreelaja Nair 9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) Karoline Holler and Jan Philipp Junker 10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos Clare E. Buckley 11. Ex Utero Culture and Imaging of Mouse Embryos Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis 12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield 13. Reptile Embryology and Regeneration Matthew K. Vickaryous and Emily A.B. Gilbert 14. Manipulation of Developmental Function in Turtles with Notes on Alligators Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas 15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero 16. Visualizing the Balbiani Body in Zebrafish Oocytes KathyAnn L. Lee and Florence L. Marlow 17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis Allison Jamieson-Lucy and Mary C. Mullins 18. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos Romain Gibeaux and Rebecca Heald 19. Germ Cell Transplantation in Avian Species Young Hyun Park, Young Min Kim, and Jae Yong Han 20. Production of Germ-Line Chimeras in Zebrafish Taiju Saito, Rie Goto, Nicola Rivers, and Etsuro Yamaha 21. A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring Zsolt Csenki and Ferenc Mueller 22. Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, and Jose B. Cibelli 23. An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish Cara E. Moravec and Francisco J. Pelegri 24. Modeling Embryonic Cleavage Patterns Dmitry Ershov and Nicolas Minc 25. Laboratory Guidelines for Animal Care Marcelo Couto and Charles Cates

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions