Overview

This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three  parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.  Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.

Full Product Details

Author:   Mark A.G. Eldridge ,  Adriana Galvan
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   195
Weight:   0.662kg
ISBN:  

9781071629208

ISBN 10:   1071629204
Pages:   333
Publication Date:   07 February 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain.- HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport.-Generation of High-Titer Defective HSV-1 Amplicon Vectors.- Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research.- Quality control for Adeno-associated viral vector production.- Vector tropism.-Viruses for systemic delivery.- Transcriptomic Definition of Neuron Types.- Enhancers for selective targeting.- Pathway-selective reversible perturbations using a double-infection technique in the macaque brain.- Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs.- Convection-enhanced delivery of viral vectors.- Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain.- Methods to verify expression and function of DREADDs using PET.- Reporter selection and post-mortem methods to verify transgene expression.- Considerations for the use of viral vectors in NHP experiments.

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