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OverviewHypermethylated In Cancer 1 (HIC1) gene was identified as a potential tumour suppressor but its physiological role has remained enigmatic. This work (master thesis) provides insights into the biological relevance of HIC1 not only in the context of Wnt signalling. The book contains summary of data on the Wnt signalling pathway and HIC1 available in the literature (current of April 2008) and very broad yet consistent set of laboratory approaches and detailed manuals. These methods can be very helpful to students interested in molecular biology. The whole work is followed by thorough discussion of the presented results. Most interestingly, one chapter of the book describes detailed strategy for generating gene targeted mouse. This is exemplified by production of two strains: (1) a strain for conditional inactivation of Hic1 gene using Cre/loxP system and (2) so-called reporter mouse strain that expresses the fluorescent protein from endogenous Hic1 promoter. A separate file containing the author's lecture 'Generation of conditional knock-out mouse step by step' is included to provide a comprehensive overview of the research method that has pervaded all fields of contemporary biology. Full Product DetailsAuthor: Vendula PospichalovaPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.70cm , Length: 22.90cm Weight: 0.177kg ISBN: 9783838361239ISBN 10: 3838361237 Pages: 112 Publication Date: 02 June 2010 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |