Transposable Elements: Methods and Protocols

Author:   Miguel R. Branco ,  Alexandre de Mendoza Soler
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2607
ISBN:  

9781071628829


Pages:   382
Publication Date:   01 December 2022
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Transposable Elements: Methods and Protocols


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Author:   Miguel R. Branco ,  Alexandre de Mendoza Soler
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2607
Weight:   0.940kg
ISBN:  

9781071628829


ISBN 10:   1071628828
Pages:   382
Publication Date:   01 December 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. An overview of best practices for transposable element identification, classification and annotation in eukaryotic genomes Fernando Rodriguez and Irina R. Arkhipova   2. Assembly-free annotation and quantification of transposable elements with dnaPipeTE Clément Goubert   3. Best practice for the identification of horizontally transferred transposons James D Galbraith, Zhipeng Qu, Atma M Ivancevic, David L Adelson   4. Genotyping of transposable elements insertions segregating in the human populations using short-read re-alignments Xun Chen, Guillaume Bourque, and Clément Goubert   5. A Pangenome approach to detect and genotype TE insertion polymorphisms Cristian Groza, Guillaume Bourque, and Clément Goubert   6. Experimental validation of transposable element insertions using the Polymerase Chain Reaction (PCR) Miriam Merenciano, Marta Coronado-Zamora, and Josefa González   7. Quantification of LINE-1 RNA expression from bulk RNA-seq using L1EM Wilson McKerrow   8. Genome-wide profiling of L1 DNA methylation by bs-ATLAS-seq Claude Philippe and Gael Cristofari   9. Nanopore epigenomic analysis of transposable element DNA modifications Nathan Smits and Geoffrey Faulkner   10. Targeted resequencing and methylation analysis of L1 elements by nanopore sequencing Arpita Sarkar, Sophie Lanciano, and Gael Cristofari   11. Inferring protein-DNA binding profiles at interspersed repeats using HiChIP and PatChER Darren Taylor and Miguel R Branco   12. Affinity-based Interactome Analysis of Endogenous LINE-1 Macromolecules Luciano H. Di Stefano, Leila Saba, Mehrnoosh Oghbaie, Hua Jiang, Wilson McKerrow, Maria Benitez-Guijarro, Martin S. Taylor, and John LaCava   13. LINE-1 retrotransposition assays in embryonic stem cells Marta Garcia-Canadas, Francisco Sanchez-Luque, Laura Sanchez, Johana Rojas, and Jose Garcia Perez   14. Detecting somatic transposable element insertions in Drosophila tissues Katarzyna Siudeja   15. Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering” Vivien M Weber, Aurelien J Doucet, and Gael Cristofari   16. Epigenetic editing of transposable and repetitive elements Joanna M Jachowicz   17. Using CRISPR to investigate the regulatory activity of transposable elements David M Simpson, Conor R Kelly, and Edward B Chuong

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