Overview

A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biologya series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Full Product Details

Author:   Leandro Peña
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2004
Volume:   286
Dimensions:   Width: 15.20cm , Height: 2.50cm , Length: 22.90cm
Weight:   0.688kg
ISBN:  

9781617374616

ISBN 10:   161737461
Pages:   437
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9781617795572
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Transgenic Plants: An Historical Perspective.- Transformation.- Plant Transformation: Agrobacterium-Mediated Gene Transfer.- Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation.- Stable Transformation of Plant Cells by Particle Bombardment/Biolistics.- Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts.- Floral Dip: Agrobacterium-Mediated Germ Line Transformation.- Production of Transgenic Crops by the Floral-Dip Method.- Chloroplast Genetic Engineering to Improve Agronomic Traits.- Regeneration.- Organogenesis From Transformed Tomato Explants.- Genetic Transformation of Conifers Utilizing Somatic Embryogenesis.- Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues.- Genetic Transformation of Mature Citrus Plants.- Selection.- Selectable Markers: Antibiotic and Herbicide Resistance.- Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection.- Green Fluorescent Protein Quantification in Whole Plants.- Positive Selection.- Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems.- Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination.- Transgene Integration, Expression, and Localization.- The Study of Transgene Copy Number and Organization.- Analysis of Gene Expression in Transgenic Plants.- Transgene Integration: Use of Matrix Attachment Regions.- Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes.- Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction.- Risk Assessment.- Agrobacterium Persistence in Plant Tissues After Transformation.- Transgene Dispersal Through Pollen.- Transgenic Crops.- Transgenic Crops: The Current and Next Generations.- Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela.

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