Overview

This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.  Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.

Full Product Details

Author:   Thomas L. Saunders
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2631
Weight:   1.063kg
ISBN:  

9781071629895

ISBN 10:   1071629891
Pages:   448
Publication Date:   31 March 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Transgenesis and Genome Engineering: A Historical Review             Lluis Montoliu   2. Practical Application of the 3Rs in Rodent Transgenesis             Thorsten Buch, Boris Jerchow, and Branko Zevnik   3. Genetic and Molecular Quality Control of Genetically Engineered Mice             Lauri Lintott and Lauryl M.J. Nutter   4. Genotyping Genome-Edited Founders and Subsequent Generation             Matthew Mackenzie, Alex Fower, Alasdair J. Allan, Gemma F. Codner, Rosie K. Bunton-Stasyshyn, and Lydia Teboul   5. ASIS-Seq: Transgene Insertion Site Mapping by Nanopore Adaptive Sampling             Charles Yu, Roger Caothien, Anna Pham, Lucinda Tam, Tuija Alcantar, Natasha Bacarro, Juan Reyes Jr., Marques Jackson, Brian Nakao, and Merone Roose-Girma   6. High Throughput Analysis of CRISPR-Cas9 Editing Outcomes in Cell and Animal Models Using CRIS.py             Shilpa Narina, Jon P. Connelly, and Shondra M. Pruett-Miller   7. Advanced Technologies and Automation in mES Cell Workflow             Charles Yu, Roger Caothien, Marques Jackson, Brian Nakao, Anna Pham, Lucinda Tam, and Merone Roose-Girma   8. Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System             Benedikt Wefers, Wolfgang Wurst, and Ralf Kühn   9. Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs             Mia Wallace, J. Michael White, Evgenea Kouranova, Zi Teng Wang, and Xiaoxia Cui   10. CRISPR/Cas9 Endonuclease Mediated Mouse Genome Editing of 1 Cell and/or 2 Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Inter Homolog Repair Mechanism             Raehum Paik and Selika Garza   11. Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes             Daniel J. Davis, Hongsheng Men, and Elizabeth C. Bryda   12. CRISMERE Chromosome Engineering in Mouse and Rat             Laurence Schaeffer, Loic Lindner, Guillaume Pavlovic, Yann Hérault, and Marie-Christine Birling   13. Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus             Daniel Biggs, Chiann-mun Chen, and Benjamin Davies   14. Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes             Masahiro Sato, Ayaka Nakamura, Marie Sekiguchi, Takashi Matsuwaki, Hiromi Miura, Channabasavaiah B. Gurumurthy, Shigeru Kakuta, and Masato Ohtsuka   15. Gene Targeting in Rat Embryonic Stem Cells             Hongsheng Men, Daniel J. Davis, and Elizabeth C. Bryda   16. Rat Embryonic Stem Cell Transgenesis             Elizabeth C. Bryda, Hongsheng Men and Barbara J. Stone   17. ­CRISPR/Cas9-Mediated Genome Editing in Zebrafish             Jeffrey G. Daniel, Xinge Yu, Allison C. Ferguson, and Jordan A. Shavit   18. Generation of Rabbit Chimeras by 8-Cell Stage Embryo Injection             Dongshan Yang, Jun Song, Jie Xu, Jifeng Zhang, and Y. Eugene Chen   19. Genome Editing in Pigs             David Preisinger, Thomas Winogrodzki, Bernhard Klinger, Angelika Schnieke, and Beate Rieblinger   20. Generation of Genome-Edited Chicken through Targeting of Primordial Germ Cells             Alewo Idoko-Akoh and Michael J. McGrew

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