Overview

Philip H. Howe and a group of well-versed experimentalists present the first major volume a collection of indispensable classic and cutting-edge TGFs assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFs to methods for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature. Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists - both expert and novice - with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFs induces and modulates physiological behavior.

Full Product Details

Author:   Philip H. Howe
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   142
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   1.000kg
ISBN:  

9780896036468

ISBN 10:   0896036464
Pages:   165
Publication Date:   17 March 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

In Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition.- Measurements of Active TGF? Generated by Culture Cells.- Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes.- Detection of TGF? in Body Fluids and Tissues.- Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components.- Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction.- Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis.- Regulation of AP-1 Activity by TGF?.- CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors.- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF?.- 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay.- Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing.- Analysis of TGF?-Inducible Apoptosis.

Reviews

This book would be most useful to a lab researcher who had not previously been studying TGF-ss. Having all the practical protocols for preparing, assaying, and studying this important molecule in a small volume could be very useful for such a researcher. -Doody's Health Science Book Review Journal

This book would be most useful to a lab researcher who had not previously been studying TGF-s. Having all the practical protocols for preparing, assaying, and studying this important molecule in a small volume could be very useful for such a researcher. - Doody's Health Science Book Review Journal

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