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OverviewSince the publication of the first edition, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This edition has been updated to provide a comprehensive guide to the methods available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Full Product DetailsAuthor: David Latchman (Department of Molecular Pathology, University College London)Publisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: No.201 Dimensions: Width: 15.00cm , Height: 2.00cm , Length: 23.00cm Weight: 0.687kg ISBN: 9780199636976ISBN 10: 0199636974 Pages: 325 Publication Date: 01 March 1999 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: To order  Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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