Overview

Expert laboratory scientists describe in detail their methodologies for constructing ribozymes designed to elucidate the role of specific genes as key routes to the development of novel therapies for a wide variety of diseases. The authors review the many sites targeted with ribozymes in various diseases and provide specific accounts of the practical techniques required for the proper use of ribozymes in these systems. Their cutting-edge protocols demonstrate how to achieve ribozyme expression in distinct cellular systems, the preparation and use of high-efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition. Therapeutic Applications of Ribozymes contains all the technical support needed to make ribozyme technology a widely used and significant methodology.

Full Product Details

Author:   Kevin J. Scanlon
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   11
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   1.870kg
ISBN:  

9780896034778

ISBN 10:   0896034771
Pages:   462
Publication Date:   23 June 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Small Efficient Hammerhead Ribozymes.- The Hairpin Ribozyme.- Molecular Manipulations of the Catalytic RNAs from the Human Hepatitis Delta Virus.- The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme.- Methods for Treating HIV by Gene Therapy Using Ribozymes.- An Expression Vector for Multiple Ribozymes.- Viral RNA Targets for Ribozymes.- Hammerhead Ribozyme-Mediated Cleavage of Hepatitis B Virus RNA.- Bovine Leukemia Virus Ribozymes.- Use of Ribozymes to Specifically Alter the MDR-1 Phenotype.- Adenoviral-Mediated Anti-H-ras Ribozyme for the Treatment of Bladder Cancer.- Tissue-Specific Delivery of an anti-H-ras Ribozyme Against Malignant Melanoma.- Ribozymes as a Novel Approach for the Treatment of Human Pancreatic carcinoma.- K-rasRibozyme for Lung Cancer.- Anti-c-erb-B-2 Ribozyme for Breast Cancer.- Ribozymes.- Preformed Ribozymes.- Folate-Polylysine-Mediated Delivery of a Multiunit Anti-BCR/ABL Ribozyme to BCR/ABL-Transformed 32D Cells.- Ribozymes Targeted Against AML1/MTG8.- Ribozyme Targeting of the Growth Factor Pleiotrophin.- Ribozyme-Mediated Inhibition of Cell Proliferation.- Inhibition of ?APP Synthesis by Ribozymes and Oligonucleotides in a Coupled Reticulocyte Transcription Translation System.- Selective Modification of Fatty Acid Synthesis Pathway by Expression of an Acetyl-CoA Carboxylase-Specific Ribozyme Gene.- In Vivo Knockout of a Tissue-Specific Gene by Synthetic Ribozymes.- Ribozyme-Mediated Downregulation of Gene Expression in Transgenic Mice.- Targeted Cleavage of RNA Using External Guide Sequences and Eukaryotic RNase P.- Application of In Vitro Selection in Design of External Guide Sequences That Direct Efficient Cleavage of RNA by RNase P.- A PCR-Based Approach to Hammerhead Ribozyme Synthesis.- Measurement of RibozymeActivity Using RNase Protection.

Reviews

. . . an impressive collection of ribozyme applications. . .a useful investment for anyone who contemplates or is actively using ribozymes for target RNA inactivations. -Nature Medicine

... an impressive collection of ribozyme applications...a useful investment for anyone who contemplates or is actively using ribozymes for target RNA inactivations. -Nature Medicine

... an impressive collection of ribozyme applications...a useful investment for anyone who contemplates or is actively using ribozymes for target RNA inactivations. - Nature Medicine

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