Overview

Edited by the inventor of PCR and two prominent experts in PCR techniques, this first comprehensive handbook on the Polymerase Chain Reaction has the most up to date methodological protocols from the world's leading laboratories. Included are exciting new techniques and enhanced methods, previously unavailable in book form, that show the novice and experienced PCR user exactly how they can optimize their results. The applications chapters are quite unique, with the foremost researchers providing not only protocols, but explaining why PCR has revolutionized their particular field. Future enhancements of PCR as well as new potential uses are discussed. Readers will learn how PCR has changed the face of diagnostic testing, cancer research, genetics, forensics, plant biology, DNA sequencing, gene therapy, and much more! Nearly forty chapters have been extensively reviewed and checked for accuracy and breadth of subject matter.

Full Product Details

Author:   Kary B. Mullis ,  R. Gibbs (Baylor College of Medicine, Houston, Texas, USA) ,  Frederick Ferre
Publisher:   Birkhauser Verlag AG
Imprint:   Birkhauser Verlag AG
ISBN:  

9783764336073

ISBN 10:   3764336072
Pages:   550
Publication Date:   February 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9780817636074
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Methodology; manipulation of DNA, K. Hayashi; cloning PCR products, M. Frohman; multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain; preparation of nucleic acids, D. Shibata; amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al; quantitation - an overview, F. Ferre et al; quantification of DNAs by the polymerase chain using an internal control, D.M. Coen; RT-PCR and mRNA quantitation, J. Chelly and A. Kahn; analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson; ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis; fluorescent detection methods for PCR analysis, M.J. Heller; enzyme-labeled oligonucleotides, E. Tu & E. Jablonski; application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough; instrumentation - an overview, C. Oste; rapid cycle DNA amplification, C.T. Wittwer et al; automating PCR, H.R. Gardner; sequencing - an overview, R.A. Gibbs; and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more.

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