Free Delivery Over $100
|
|
|||
|
||||
OverviewThe fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3' -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5' -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin dromic nucleotide sequence at the 3' -end that allows the formation of a hairpin structure which provides the needed free 3'-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specific nicking of one of the strands of a circular double-stranded DNA, producing a 3'-OH group available for elongation (KORNBERG 1980). Full Product DetailsAuthor: G. Hobom , Rudolf RottPublisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K Edition: Softcover reprint of the original 1st ed. 1988 Volume: 136 Dimensions: Width: 17.00cm , Height: 0.60cm , Length: 24.20cm Weight: 0.195kg ISBN: 9783642731174ISBN 10: 3642731171 Pages: 90 Publication Date: 10 December 2011 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsRetroregulation of Bacteriophage ? int Gene Expression.- Tail Length Determination in Double-Stranded DNA Bacteriophages.- Single-Stranded DNA Phage Origins.- Initiation of DNA Replication by Primer Proteins: Bacteriophage ø29 and Its Relatives.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
||||
