Overview

This volume describes a range of methods used in complement laboratories and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Lubka T. Roumenina
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2227
Weight:   0.537kg
ISBN:  

9781071610183

ISBN 10:   107161018
Pages:   267
Publication Date:   15 April 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. The Benefits of Complement Measurements for the Clinical Practice Anne Grunenwald and Lubka T. Roumenina   2. Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source Seline A. Zwarthoff, Simone Magnoni, Piet C. Aerts, Kok P. M. van Kessel, and Suzan H.M. Rooijakkers   3. Quantification of Complement Proteins with Special Reference to C1q: Multiplex vs ELISA vs Rocket Immunoelectrophoresis vs Nephelometry Kerstin Sandholm, Barbro Persson, Sliva Abdalla, Camilla Mohlin, Bo Nilsson, and Kristina N Ekdahl   4. C3dg Quantification by PEG-precipitation and ELISA or TRIFMA Anne Troldborg and Jens Christian Jensenius   5. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope Based Enzyme-Linked Immunosorbent Assay Per H. Nilsson, Kristin Pettersen, Martin Oppermann, Espen Skjeflo, Hilde Fure, Dorte Christiansen, and Tom Eirik Mollnes   6. Sheep Erythrocytes Preparation for Hemolytic Tests Exploring Complement Functional Activities Melchior Chabannes, Pauline Bordereau, Paula Vieira Martins, and Marie-Agnès Dragon-Durey   7. Hemolytic Tests Exploring Factor H Functional Activities Melchior Chabannes, Shambhuprasad K Togarsimalemath, and Marie-Agnès Dragon-Durey   8. Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity Marloes A.H.M. Michels, Nicole C.A.J. van de Kar, Elena B. Volokhin, and Bert (L) P.W.J. van den Heuvel 9. Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry Idris Boudhabhay, Anne Grunenwald, and Lubka T. Roumenina   10. Anti-C1q Auto-Antibodies: Standard Quantification and Innovative ELISA Kinga Csorba, Lucias Schirmbeck, Denise Dubler, and Marten Trendelenburg   11. Anti-C1-Inhibitor Auto-Antibodies Detection by ELISA Chiara Suffritti, Sonia Caccia, Silvia Berra, Debora Parolin, and Marco Cicardi   12. Anti-ficolin-2 and anti-ficolin-3 Autoantibodies Detection by ELISA Chantal Dumestre-Pérard  and Nicole M. Thielens   13. Detection of anti-C3b Autoantibodies by ELISA Maria Radanova, Lubka Roumenina, and Vasil Vasilev   14. Detection of Complement Factor B Autoantibodies by ELISA Mihály Józsi and Barbara Uzonyi   15. Detection of C3 Nephritic Factor by Hemolytic Assay Melchior Chabannes, Véronique Frémeaux-Bacchi, Sophie Chauvet   16. Detection of Genetic Rearrangements inthe Regulators of Complement Activation RCA Cluster by High Throughput Sequencing and MLPA Jesús García-Fernández, Susana Vilches-Arroyo, Leticia Olavarrieta, Julián Pérez-Pérez, and Santiago Rodríguez de Córdoba   17. Complement Detection in Mouse Kidneys by Immunofluorescence Jennifer Laskowski and Joshua M. Thurman   18. Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence Marie V. Daugan, Margot Revel, Laetitia Lacroix, Catherine Sautès-Fridman, Wolf H. Fridman, and Lubka T. Roumenina   19. Analysis Of The Ligand Recognition Specificities Of Human Ficolins Using Surface Plasmon Resonance Nicole M. Thielens, Evelyne Gout, Monique Lacroix, Jean-Baptiste Reiser, and Christine Gaboriaud   20. Methods for Assessment of Interactions of Proteins with Heme - Application for Complement Proteins and Immunoglobulins Margot Revel and Jordan D. Dimitrov   21. Evaluation of Binding Kinetics and Thermodynamics of Antibody-antigen Interactions and Interactions Involving Complement Proteins Sofia Rossini and Jordan D. Dimitrov   22. Purification of Human Complement Component C4 and Sample Preparation for Structural Biology Applications Alessandra Zarantonello, Sofia Mortensen, Nick S. Laursen, and Gregers R. Andersen  

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