Overview

This is a thoroughly updated new edition which takes into account all of the many recent advances in the baculovirus expression vector system, now one of the most popular eukaryotic expression systems used. This manual is aimed at any researchers using the system in academic or industrial laboratories, and can be used by those new to the technique as well as those experienced with these methods.

Full Product Details

Author:   L.A. King ,  R.D. Possee
Publisher:   Chapman and Hall
Imprint:   Chapman and Hall
Edition:   2nd Revised edition
ISBN:  

9780412755507

ISBN 10:   0412755505
Pages:   304
Publication Date:   31 July 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The baculovirus; the baculovirus expression system; processing of foreign proteins; construction of transfer vectors; insect cell culture media and maintenance of insect cell lines; virus propagation and purification; production and selection of recombinant virus using linearized virus DNA; production and selection of recombinant virus in bacteria; production and selection of recombinant virus in yeast; characterization of recombinant virus; scale up of recombinant production; propagation in insect larvae; expression of foreign genes in stable, continuous insect lines; trouble shooting guide.

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