Overview

Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition (see page 22). This video guide complements the above mentioned manual and Transgenic Techniques in Mice: A Video Guide (see below). It provides step-by-step demonstrations of the following procedures required for making mutant mice. Demonstration 1: Starting embryonic stem (ES) cell lines Demonstration 2: Making fibroblast feeder layers Demonstration 3: Growing established ES cell lines Demonstration 4: Targeting ES cell lines Demonstration 5: Making chimeras with ES cells Demonstration 6: Making a mouse

Full Product Details

Author:   Roger A. Pederson (University of California, San Francisco, USA) ,  Virginia Papaioannou (Columbia University, USA) ,  Alexandra Joyner (Mount Sinai Hospital, USA and University of Toronto, Canada) ,  Janet Rossant (Mount Sinai Hospital, USA and University of Toronto, Canada)
Publisher:   Cold Spring Harbor Laboratory Press,U.S.
Imprint:   Cold Spring Harbor Laboratory Press,U.S.
Dimensions:   Width: 12.10cm , Height: 3.00cm , Length: 20.30cm
Weight:   0.259kg
ISBN:  

9780879694302

ISBN 10:   0879694300
Publication Date:   01 January 1993
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Video
Publisher's Status:   Active
Availability:   In Print  
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Language:   English

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