Overview

The initial incentive for in-depth study of Simian Virus 40 biology originated from the revelation of public health implications its use had in the 1960s. SV40 gained notoriety at this time when it was found to be a contaminant of polio and adenovirus vaccines that had been distributed to millions of healthy individuals worldwide. SV40 Protocols presents a consolidation of numerous well-tested step-by-step techniques from experts with hands-on experience. The book is organized in two sections. The first relates to assays of the lytic cycle of the virus, starting with a valuable introductory chapter on basic techniques for growth of SV40 and its mutants in tissue culture. Techniques for the growth and detection of two SV40-related human viruses, BKV and JCV, are also presented for those involved in clinical research. The second section begins with detailed biological assays of neoplastic transformation that are applicable to any system. All of the detailed and thorough chapters include discussions of problems and pitfalls that may be encountered, as well as troubleshooting advice. This comprehensive volume of optimzied experimental protocols will assist the beginner, as well as more experience researchers, to over come problems of ambiguity often present in SV40 literature.

Full Product Details

Author:   Leda Raptis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   165
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   1.440kg
ISBN:  

9780896036536

ISBN 10:   0896036537
Pages:   310
Publication Date:   26 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
We will order this item for you from a manufatured on demand supplier.

Table of Contents

Propagation of Wild-Type and Mutant SV40.- Propagation and Assay of the JC Virus.- Propagation and Assay of BK Virus.- Molecular Methods for Identification and Genotyping of BK Virus.- Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication.- Cell-Free Transcription of SV40.- Quantitation and Structural Analysis of SV40 RNAs.- Generation of Recombinant SV40 Vectors for Gene Transfer.- Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions.- Using Retroviral Vectors to Express SV40 Tumor Antigens.- Neoplastic Transformation Assays.- Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen.- SV40-Mediated Immortalization.- Biology of p53 and SV40 Large T Association.- Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins.- In situ Electroporation for the Measurement of c-Ras Activation by SVLT.- Role of SV40 Small t in Cell Lysis, Transformation, and Signaling.- Cytotoxic T Lymphocytes in SV40 Infections.- Detection of SV40 DNA Sequences in Human Tissue.- Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen.

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