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OverviewMany investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles. Full Product DetailsAuthor: J. M. Graham (, Merseyside Innovation Centre, Liverpool) , D. Rickwood (Department of Biology, Department of Biology, University of Essex)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 173 Dimensions: Width: 15.60cm , Height: 2.00cm , Length: 23.40cm Weight: 0.617kg ISBN: 9780199634941ISBN 10: 0199634947 Pages: 360 Publication Date: 30 January 1997 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: To order Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of Contents1. Homogenization of tissues and cells ; 2. Isolation of subcellular fragments ; 3. Isolation and characterization of nuclei and nuclear subfractions ; 4. Subcellular fractionation of mitochondria ; 5. Isolation and characterization of peroxisomes ; 6. Lysosomes and endocytosis ; 7. The membranes of the secretory and exocytic pathways ; 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells ; 9. Ribosomes and polysomes ; 10. Electron microscopy of organellesReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |