Subcellular Fractionation: A Practical Approach

Author:   J. M. Graham (, Merseyside Innovation Centre, Liverpool) ,  D. Rickwood (Department of Biology, Department of Biology, University of Essex)
Publisher:   Oxford University Press
Volume:   173
ISBN:  

9780199634941


Pages:   360
Publication Date:   30 January 1997
Format:   Paperback
Availability:   To order   Availability explained
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Subcellular Fractionation: A Practical Approach


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Overview

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Full Product Details

Author:   J. M. Graham (, Merseyside Innovation Centre, Liverpool) ,  D. Rickwood (Department of Biology, Department of Biology, University of Essex)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   173
Dimensions:   Width: 15.60cm , Height: 2.00cm , Length: 23.40cm
Weight:   0.617kg
ISBN:  

9780199634941


ISBN 10:   0199634947
Pages:   360
Publication Date:   30 January 1997
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   To order   Availability explained
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1. Homogenization of tissues and cells ; 2. Isolation of subcellular fragments ; 3. Isolation and characterization of nuclei and nuclear subfractions ; 4. Subcellular fractionation of mitochondria ; 5. Isolation and characterization of peroxisomes ; 6. Lysosomes and endocytosis ; 7. The membranes of the secretory and exocytic pathways ; 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells ; 9. Ribosomes and polysomes ; 10. Electron microscopy of organelles

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