Overview

This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.

Full Product Details

Author:   Federico Iovino
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1968
Weight:   0.695kg
ISBN:  

9781493991983

ISBN 10:   1493991981
Pages:   227
Publication Date:   01 April 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Editor Biography...Preface...Table of Contents...Contributing Authors... Part I Cultivation of Streptococcus pneumoniae In Vitro 1. Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid MediaEsther Texeira and Norma Suarez Part II Microscopy Techniques to Study the Biology of Streptococcus pneumoniae and Pneumococcal Interactions with the Host 2. Electron Microscopy to Study the Fine Structure of the Pneumococcal CellSven Hammerschmidt and Manfred Rohde 3. Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal CellFederico Iovino and Birgitta Henriques-Normark 4. Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the ChromosomeMorten Kjos 5. High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the HostFederico Iovino and Birgitta Henriques-Normark Part III The Genetics of Streptococcus pneumoniae 6. Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal ChromosomeIsabelle Mortier-Barriere, Nathalie Campo, Mathieu Berge, Marc Prudhomme, and Patrice Polard 7. Gene Expression Analysis in the PneumococcusRory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, Luisa N. Hiller 8. Transcriptional Knockdown in Pneumococci using CRISPR InterferenceMorten Kjos Part IV The Proteome and Proteomics of Streptococcus pneumoniae 9. Protein Expression Analysis by Western Blot and Protein-Protein InteractionsMaria Dolores Cima-Cabal, Fernando Vazquez, Juan R. de los Toyos, and Maria del Mar Garcia-Suarez 10. Mass Spectrometry to Study the Bacterial Proteome from a Single ColonyJianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng 11. Bead-Based Flow Cytometric Cell Counting of Live and Dead BacteriaFang Ou, Cushla McGoverin, Joni White, Simon Swift, and Frederique Vanholsbeeck Part V Streptococcus pneumoniae-Host Interactions: In Vitro and In Vivo Models 12. In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host CellsTerry Brissac and Carlos J. Orihuela 13. Growing and Characterizing Biofilms Formed by Streptococcus pneumoniaeYashuan Chao, Caroline Bergenfelz, and Anders P. Hakansson 14. In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal DiseaseVicky Sender, Birgitta Henriques-Normark, and Federico Iovino 15. Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal InfectionMiguel Palomino-Segura and Santiago F. Gonzalez 16. IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In VivoAdam Sierakowiak, Birgitta Henriques-Normark, and Federico Iovino Part VI Public Health, Epidemiology, and Biostatistics 17. The Pneumococcus and Its Critical Role in Public HealthGodwin Oligbu, Norman K. Fry, and Shamez Ladhani 18. The Epidemiology and Biostatistics of PneumococcusGodwin Oligbu, Norman K. Fry, and Shamez Ladhani

Reviews

The audience includes students, researchers, and microbiologists, as well as others in the fields of public health, epidemiology, and biostatistics related to Streptococcus pneumoniae. ... This is an excellent book on Streptococcus pneumoniae, with additional coverage of the related public health, epidemiology, and biostatistics aspects. I am sure that the information in this book will be of much help and interest to the intended audience. (Omer Iqbal, Doody's Book Reviews, December 7, 2019)

“The audience includes students, researchers, and microbiologists, as well as others in the fields of public health, epidemiology, and biostatistics related to Streptococcus pneumoniae. ... This is an excellent book on Streptococcus pneumoniae, with additional coverage of the related public health, epidemiology, and biostatistics aspects. I am sure that the information in this book will be of much help and interest to the intended audience.” (Omer Iqbal, Doody's Book Reviews, December 7, 2019)

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