Overview

Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.  Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.

Full Product Details

Author:   Kursad Turksen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2013
Volume:   976
Dimensions:   Width: 17.80cm , Height: 1.00cm , Length: 25.40cm
Weight:   3.714kg
ISBN:  

9781493963034

ISBN 10:   1493963031
Pages:   179
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.
Language:   English

Table of Contents

Comprehensive Hematopoietic Stem Cell Isolation Methods.- Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo.- Mouse Hematopoietic Stem Cell Transplantation.- Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells.- Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces.- Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy.- Cardiac Stem Cell Senescence.- Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions.- Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells.- Intra-Femoral Injection of Human Mesenchymal Stem Cells.- Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency.- Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging.- Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue.

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