Overview

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

Full Product Details

Author:   Gerd Klein ,  Patrick Wuchter
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2017
Weight:   0.500kg
ISBN:  

9781493995769

ISBN 10:   1493995766
Pages:   248
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Evolution of Peripheral Blood Stem Cell Transplantation             Anthony D. Ho   2. In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents             Motomu Tanaka   3. Colony Formation: An Assay of Hematopoietic Progenitor Cells             Romy Kronstein-Wiedemann and Torsten Tonn   4. Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking             Katharina Kriegsmann and Patrick Wuchter   5. Transmigration Assays for the Determination of Molecular Interactions between Hematopoietic Stem Cells and Niche Cells             Reinhard Henschler and Rudolf Richter   6. Microfluidic Shear Force Assay to Determine Cell Adhesion Forces             Julia Hümmer, Julian Koc, Axel Rosenhahn, and Cornelia Lee-Thedieck   7. A Microcavity Array-Based 3D-Model System of the Hematopoietic Stem Cell Niche             Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, and Stefan Giselbrecht   8. Migration Assay for Leukemic Cells in a 3D Matrix toward a Chemoattractant             Sabrina Zippel, Annamarija Raic, and Cornelia Lee-Thedieck   9. Intravital Imaging of Blood Flow and HSPC Homing in Bone Marrow Microvessels             Jonas Stewen and Maria Gabriele Bixel   10. Assessing Cellular Hypoxic Status In Situ within the Bone Marrow Microenvironment             Ute Suessbier and César Nombela-Arrieta   11. Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry             Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, and Jochen Guck   12. Assessment of Proteolytic Activities in the Bone Marrow Microenvironment             Andreas Maurer, Gerd Klein, and Nicole D. Staudt   13. Analysis of the Complement Cascade Activation during Mobilization of Hematopoietic Stem/Progenitor Cells             Anna I. Grabowska and Jakub M. Hawryluk   14. A Freezing Protocol for Hematopoietic Stem Cells             Petra Pavel and Sascha Laier   15. Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays             Yu Wei Zhang and Nina Cabezas Wallscheid   16. Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells             Martina Konantz, Joëlle S. Müller, and Claudia Lengerke   17. Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells             Walter de Back, Thomas Zerjatke, and Ingo Roeder

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