Overview

Statin is a potential antihypercholesterolemic agent, which inhibits 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate limiting enzyme of cholesterol biosynthesis. The beneficial effects of statin therapy, however, are not limited to patients with hypercholesterolemia. Increasing evidence suggest that an activity level of HMG-CoA reductase may affect various cellular functions such as DNA synthesis, cell proliferation, diverse signal transduction pathways leading to pathogenesis. The use of HMG-CoA reductase inhibitor in varying diseases is under investigation. This book is the most current hands-on book in the field of hepatocarcinogenesis. This book provides valuable coverage in hepatocellular carcinoma. It offers the latest techniques and approaches to conduct the hepatocarcinogenesis studies such as cell proliferation and differentiation. In the present study, the authors have evaluated the chemo-preventive potential of statins as the end point biomarker in a diethylnitrosamine-induced rat hepatocarcinogenesis model by morphometric analysis of precancerous lesions during neoplastic transformation of cells. Further, hepatic histopathological assessments of preneoplastic lesions are carried out and correlated with the morphometric findings. Expression of proliferating cell nuclear antigen (PCNA), Bromodeoxyuridine (BrdU) and metallothionein (MT) are studied by enzyme labeled immunochemistry.

Full Product Details

Author:   Prafulla Kumar Sahu ,  Muthumani Janarthan ,  Dilip Kumar Sahoo
Publisher:   Springer, India, Private Ltd
Imprint:   Springer, India, Private Ltd
Volume:   1
ISBN:  

9788132208662

ISBN 10:   8132208668
Pages:   112
Publication Date:   31 July 2013
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   In stock  
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Table of Contents

1.Introduction.- 1.1.Carcinogenesis.- 1.2.Pathology of Liver Cancer.- 1.3.Cancer chemoprevention.- 1.4.Statin .- 1.5.Histopathological and Biochemical Assay.- 1.6.Placental Glutathione S-Transferase Positive Foci.- 1.7.Cell Proliferation.- 1.8.Metallothionein.- 1.9.Objectives of the Present Work.- 2.Materials and Methods.- 2.1.Hepatic Histomorphometry and Histochemistry.- 2.2.Expression of Placental Glutathione S Transferasepositive (GST-P).- 2.3.Cell Proliferation Assay. 2.4.Metallothionein.- 3.Results and Discussion.- 3.1.Effect of Statin on Body and Liver Weights in rat treated with DEN + PB.- 3.2.Effect of Statin on the Number and Size Distribution of Visible Hyperplastic Nodules in Rat Liver Treated with DEN+PB.- 3.3.Effect of Statin on Hepatic Histopathology.- 3.4.Effect of Statin on the Induction of GGT-Positive Foci in Rats Treated with DEN + PB .- 3.5.Effect of Statin on the Induction of PAS-Reaction and Alkaline Phosphatase in Rats Treated with DEN + PB.- 3.6.Effect of Statin on the impression of GST-P Positive Foci Rat Liver Treated with DEN + PB.- 3.7.Effect of Statin on DEN + PB induced PCNA expression in Rat Liver.- 3.8.Effect of Statin on DEN + PB induced BrdU-Li in the Rat Liver .- 3.9.Effect of Statin on DEN + PB induced Metallothionein overexpression.- 4.Summary and Conclusions.- 5.Bibliography.

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Author Information

Dr. Prafulla Kumar Sahu has received his B.Pharm degree from Rajiv Gandhi University of Health Sciences, Bengaluru and M.Pharm degree from Biju Patnaik University of Technology. He did his Ph.D. from Berhampur University, where he gained an appreciation of excitement of research from his mentor, Prof. M. M. Annapurna. He began his career as a faculty in Roland Institute of Pharmaceutical Sciences, where he developed an interest in pharmaceutical analysis and bioanalytical chemistry. Now he is working as a professor, head of department at Raghu College of Pharmacy, Visakhapatnam, Andhra Pradesh, India. Dr. Sahu has more than 20 national/ international peer reviewed research articles in high impact journals. He has authored 2 books in 2010 and 2012, respectively, and has served as a reviewer in many international journals. Dr. Janarthan Muthumani is a professor at NIMRA College of Pharmacy. He received his Bachelor of Pharmacy degree (1995) from Tamil Nadu Dr. MGR Medical University and his Master degree in Pharmacology (1997) from Bangalore University. He obtained his Doctor of Philosophy in Pharmacy from Jadavpur University Kolkata under the supervision of Professor Malay Chatterjee. He has to his credit over seven research papers in international journals and two book chapters. He trains and assists students and researchers across pharmacy disciplines in designing, conducting and analyzing research projects. Dr. Dillip Kumar Sahoo, M.Pharm., Ph.D., possesses over eight years of experience in teaching the subject of Pharmaceutical Chemistry to students of B. Pharmacy and M. Pharmacy. He has published nine research papers in national and international journals. He is the recipient of Meritorious Teachers Award from Jawaharlal Nehru Technological University, Kakinada for the year 2010.

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