Overview

This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Full Product Details

Author:   Marc G. Dumont ,  Marcela Hernández García
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2046
Weight:   0.500kg
ISBN:  

9781493997237

ISBN 10:   1493997238
Pages:   247
Publication Date:   13 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Experimental Set Up and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era             Roey Angel   2. DNA-Based Stable Isotope Probing             Zhongjun Jia, Weiwei Cao, and Marcela Hernández García   3. RNA Stable Isotope Probing (RNA-SIP)             Noor-Ul-Huda Ghori, Benjamin Moreira-Grez, Paton Vuong, Ian Waite, Tim Morald, Michael Wise, and Andrew S. Whiteley   4. Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples             Andrea Watzinger and Rebecca Hood-Nowotny   5. SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity             Martin Taubert   6. Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS             Xavier Mayali, Peter K. Weber, Erin Nuccio, Jory Lietard, Mark Somoza, Steven J. Blazewicz, and Jennifer Pett-Ridge   7. Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures             Quan Yuan   8. Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmental Microbes             Li Cui, Kai Yang, and Yong-Guan Zhu   9. Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High Resolution SIP             Samuel E. Barnett, Nicholas D. Youngblut, and Daniel H. Buckley   10. Stable Isotope Probing of Microorganisms in Environmental Samples with H218O             Egbert Schwartz, Michaela Hayer, Bruce A. Hungate, and Rebecca L. Mau   11. Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing             Brianna K. Finley, Michaela Hayer, Rebecca L. Mau, Alicia M. Purcell, Benjamin J. Koch, Natasja van Gestel, Egbert Schwartz, and Bruce A. Hungate   12. Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics             Eileen Kröber and Özge Eyice   13. Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing             Ang Hu, Yahai Lu, Marcela Hernández García, and Marc G. Dumont   14. Stable Isotope Probing Techniques and Methodological Considerations Using 15N             Roey Angel   15. DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea             Xiuran Yin, Ajinkya C. Kulkarni, and Michael W. Friedrich   16. Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethane Biodegradation             Miao Hu, Matthew Lee, Ling Zhong, and Michael J. Manefield   17. RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment             Severin Weis, Sylvia Schnell, and Markus Egert   18. Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere             Wafa Achouak and Feth el Zahar Haichar

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions