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Author:   Yasuto Murayama ,  Yasutaka Kakui
Publisher:   Springer-Verlag New York Inc.
Imprint:   Humana
ISBN:  

9781071650042

ISBN 10:   1071650041
Pages:   276
Publication Date:   20 October 2025
Audience:   General/trade ,  General
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Single-Molecule Analyses of Human Cohesin Complex.- Interaction between an Individual Cohesin Complex and DNA Revealed by Optical Tweezers and Single-Molecule Microscopy.- Visualising Binding of Single SMC Complexes to DNA Substrates Using Combinational Optical Tweezers and Fluorescence Microscopy.- Atomic Force Microscopy Analysis of Bridging-Induced Phase Separation Mediated by SMC Proteins.- Monitoring Local Chromatin Dynamics Regulated by SMC Complex in Fission Yeast.- DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.- Gel Electrophoresis Analysis of SMC-Mediated Effects on DNA Topology.- The Use of the Homobifunctional Crosslinker BMOE to Investigate Cohesin Function by Measuring Protein-Protein and Protein-DNA Interactions.- Bulk FRET Analysis of the Conformation of the Cohesin Ring.- Assessing SMC Complex Function in Replication Fork Progression with DNA Fiber Assays.- Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation.- A Biochemical Assay to Monitor the Encounter between Cohesin and DNA Replication.- An In Vitro Assay to Study RSC Remodeling Activity Stimulation by the Cohesin Loader.- Purification and In Vitro Analysis of Escherichia Coli RecN.- Production and Purification of the Human SMC5/6 Core Complex from S. cerevisiae.- ChIA-PET Analysis for Investigating SMC-Mediated 3D Genomic Contacts.- High-Resolution Chromosome Conformation Capture Method to Study Archaeal SMC Complexes.

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