Overview

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on  troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Full Product Details

Author:   Mathieu Rederstorff
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2300
Weight:   0.537kg
ISBN:  

9781071613887

ISBN 10:   107161388
Pages:   270
Publication Date:   02 April 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

  PART I INTRODUCTION TO SMALL NON-CODING RNAS   1 Regulatory Non-coding RNAs: An Overview Camille Virciglio, Yoann Abel, and Mathieu Rederstorff   2 RNA Precipitation Valérie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff   3 Quantitative and Qualitative Assessment of Small RNA Preparations Virginie Marchand, Lilia Ayadi, Valérie Bourguignon-Igel, and Yuri Motorin   4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant     PART II DETECTION OF SMALL NON-CODING RNAS   5 Northern Blot Detection of Tiny RNAs Jana C. Wiegard, M. Amri C. Schlüter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grünweller, and Roland K. Hartmann   6 Stem-loop qRT-PCR Based Quantification of miRNAs Yoann Abel and Mathieu Rederstorff   7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation Marlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud   8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs Valentin Vautrot, Géraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant     PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS     9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo Layea Baldini and Stéphane Labialle   10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography Aymeric Sanchez and Sylvain Maenner   11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) Charbel Alfeghaly, Isabelle Behm-Ansmant, and Sylvain Maenner   12 Gene Reporter Assays to Study miRNA Function Yoann Abel and Mathieu Rederstorff   13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo Bin Yi, Kristina Larter, and Yaguang Xi     PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS     14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs Quentin Thuillier and Isabelle Behm-Ansmant   15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma Virginie Marchand, Adeline Galvanin, and Yuri Motorin   16 Analysis of the RNA and protein complexome by Grad-seq Jens Hör and Jörg Vogel   17 µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs Farah Bouhedda, Roger Cubi, Stéphanie Baudrey and Michael Ryckelynck     PART V STRUCTURAL APPROACHES     18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT Fatima Alghoul, Gilbert Eriani, and Franck Martin   19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions