|
|
|||
|
||||
OverviewThe ability of double-stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done. Full Product DetailsAuthor: Gregory J. HannonPublisher: Cold Spring Harbor Laboratory Press,U.S. Imprint: Cold Spring Harbor Laboratory Press,U.S. Edition: 2nd Revised edition Dimensions: Width: 21.60cm , Height: 2.00cm , Length: 29.00cm Weight: 1.315kg ISBN: 9780879697044ISBN 10: 0879697040 Pages: 436 Publication Date: 01 August 2003 Audience: General/trade , College/higher education , Professional and scholarly , General , Undergraduate Format: Paperback Publisher's Status: Out of Print Availability: Out of stock Table of ContentsChapter 1. Sense Cosuppression in Plants: Past, Present, and Future Richard A. Jorgensen Chapter 2. Transgene Cosuppression in Animals James A. Birchler, Manika Pal-Bhadra, and Utpal Bhadra Chapter 3. Regulation of the Genome by Double-stranded RNA Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke Chapter 4. RNAi in Caenorhabditis elegans Ren e9; F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk Chapter 5. Small RNAs, Big Biology: Biochemical Studies of RNA Interference Gregory J. Hannon and Phillip D. Zamore Chapter 6.shRNA-mediated Silencing of Mammalian Gene Expression Michael T. McManus and Douglas S. Conklin Chapter 7. Transposable Elements, RNA Interference, and the Origin of Heterochromatin Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, and Vincent Colot Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing Allen W. Nicholson Chapter 9. RNA-dependent RNA Polymerase in Gene Silencing Luyun Huang, John Gledhill, and Craig E. Cameron Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization Ira M. Hall and Shiv I.S. Grewal Chapter 11. Total Interference: Genome-wide RNAi Screens in C. elegans Andrew G. Fraser and Julie Ahringer Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants Tessa M. Burch-Smith, Jennifer L. Miller, and S.P. Dinesh-Kumar Chapter 13. Mammalian RNA Interference Thomas Tuschl Chapter 14. RNAi in Avian Embryos Esther T. Stoeckli Chapter 15. Guide to RNAi in Mouse Oocytes and Preimplantation Embryos Paula Stein and Petr Svoboda Chapter 16. RNAi Technologies in Drosophila Cell Culture Scott M. Hammond Chapter 17. RNAi Applications in Drosophila melanogaster Richard W. Carthew Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms Elisabetta Ullu and Christian TschudiReviewsBecause of the overwhelming plethora of studies, this book, written by leaders in the field, arrives as a welcome road map for beginners and experts alike. The volume provides a broad overview of the basic mechanisms and applications of RNAi to different model organisms for functional gene analysis, including nonclassical model systems such as the parasitic protozoan Trypanosoma brucei and the chicken embryo. In addition, several chapters are devoted to subjects that are more loosely related to RNAi and thus less frequently reviewed in papers in the field...RNAi is indeed obligatory reading for anyone interested in modern molecular biology. Science This book provides a useful and relatively comprehensive summary of progress in the field, and it is likely that every biologist will find something of interest. While experts in this field will be able to update their knowledge on RNAi and its applications (for example, its use in high-throughput screening), the book should also serve as a useful introduction to the world of small RNAs for the non-expert. An added bonus is the inclusion of many protocols that explain the details of key RNAi experiments that once again should prove useful to the expert and non-expert alike. Nature Cell Biology Author InformationTab Content 6Author Website:Countries AvailableAll regions |