Overview

RNA Silencing: Methods and Protocols facilitates the translation of gene silencing concepts into practical applications, and includes a broad and useful set of RNA silencing protocols. Sections cover the biochemical aspects of silencing machinery, methods for RNA silencing in nonmammalian organisms, design, preparation, and use of RNAs to silence gene expression, several methos for the in vivo delivery of siRNAs and silencing vectors, and methods for the study and use of microRNAs.

Full Product Details

Author:   Gordon Carmichael
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2005 ed.
Volume:   309
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.678kg
ISBN:  

9781588294364

ISBN 10:   1588294366
Pages:   338
Publication Date:   01 June 2005
Audience:   General/trade ,  General
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method.- Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis.- Preparation and Analysis of Drosha.- RNA Interference in Caenorhabditis elegans.- Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei.- Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing.- Strategies for the Design of Random siRNA Libraries and the Selection of anti-GFP siRNAs.- Silencing Gene Expression with Dicer-Generated siRNA Pools.- Inhibition of Gene Expression In Vivo Using Multiplex siRNA.- Gene Silencing by a DNA Vector-Based RNAi Technology.- Plasmid-Based RNA Interference.- siRNA Delivery In Vivo.- Peptide-Based Strategy for siRNA Delivery into Mammalian Cells.- Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells.- Exon-Specific RNA Interference.- Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs.- Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro.- High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes.- Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA.

Reviews

From the reviews: <p> RNA Silencing: Methods and Protocols is a comprehensive and user-friendly reference and worth recommending. Researchers who are interested in using siRNA as a major method to do gene knockdown experiments in vitro to study gene function instead of using time-consuming gene targeting in mice and those who are interested in studying the functions of genes in the development of cell defense against viruses will find this book an excellent resource. (Weimin He, In Vitro Cellular & Developmental Biology - Animal, Vol. 42, May and June, 2006)

From the reviews: RNA Silencing: Methods and Protocols is a comprehensive and user-friendly reference and worth recommending. Researchers who are interested in using siRNA as a major method to do gene knockdown experiments in vitro to study gene function instead of using time-consuming gene targeting in mice and those who are interested in studying the functions of genes in the development of cell defense against viruses will find this book an excellent resource. (Weimin He, In Vitro Cellular & Developmental Biology - Animal, Vol. 42, May and June, 2006)

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