RNA Scaffolds: Methods and Protocols

Author:   Luc Ponchon
Publisher:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2323
ISBN:  

9781071614983


Pages:   288
Publication Date:   05 June 2021
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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RNA Scaffolds: Methods and Protocols


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Author:   Luc Ponchon
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2323
Weight:   0.772kg
ISBN:  

9781071614983


ISBN 10:   1071614983
Pages:   288
Publication Date:   05 June 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA.- RNA Footprinting Using Small Chemical Reagents.- Improving RNA Crystal Diffraction Quality by Post-Crystallization Treatment.- Using tRNA Scaffold to Assist RNA Crystallization.- RNA Modeling with the Computational Energy Landscape Framework.- Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli.- In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold.- Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds.- Identification of RNA-Binding Proteins Associated to RNA Structural Elements.- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.- Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging.- Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria.- FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers.- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach.- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells.- Folding RNA–Protein Complex into Designed Nanostructures.- An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.- Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs.- Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics.

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