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OverviewIn this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA. Full Product DetailsAuthor: Frank J. SchmidtPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2015 ed. Volume: 1240 Dimensions: Width: 17.80cm , Height: 1.40cm , Length: 25.40cm Weight: 6.377kg ISBN: 9781493918959ISBN 10: 1493918958 Pages: 219 Publication Date: 29 October 2014 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand We will order this item for you from a manufactured on demand supplier. Table of ContentsIdentifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique.- RNA-directed Recombination of RNA in Vitro.- RNA-RNA Selex.- Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System.- Trans-activation of Large Ribozymes.- Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies.- Single Molecule Studies of RNA-RNA Interactions.- Modification Interference Analysis of the Ribosome.- Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping.- Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA.- Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.- Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by Small Angle X-ray Scattering.- Fusion RNAs in Crystallographic Studies of Double-stranded RNA from Trypanosome RNA Editing.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |