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OverviewRNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. Full Product DetailsAuthor: Christopher W.J. Smith (Lecturer in Biochemistry, University of Cambridge)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 192 Dimensions: Width: 15.70cm , Height: 2.00cm , Length: 23.50cm Weight: 0.625kg ISBN: 9780199636501ISBN 10: 0199636508 Pages: 366 Publication Date: 09 July 1998 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of Contents1: Applications of chemically synthesized RNA 2: Preparation of RNA-Protein complexes for X-ray crystallography and NMR 3: Uses of site-specifically modified RNAs constructed by RNA ligation 4: The Electrophoretic Mobility Shift Assay for RNA Binding Proteins 5: Affinity methods for isolating RNA-binding proteins 6: Synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing 7: Detecting RNA-Protein Interactions and Isolating cDNA Clones by Northwestern Screening 8: A three-hybrid system to detect and analyse RNA-protein interactions in vivo 9: In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay 10: Foot-printing and modification-interference analysis of binding sites on RNA 11: Analysis of RNA-Protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques 12: In vitro selection of nucleic acid ligandsReviewsThere are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover.--The Quarterly Review of Biology <br> There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --The Quarterly Review of Biology<br> There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --TheQuarterly Review of Biology There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --The Quarterly Review of Biology<br> Author InformationDr Christopher W J Smith, Lecturer in Biochemistry, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Old Addenbrooke's Site, Cambridge CB2 1GA. Tel: 01223 333655 / 333665. Fax: 01223 766002. Email: cwjs1@mole.bio.cam.ac.uk Tab Content 6Author Website:Countries AvailableAll regions |
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