Overview

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.

Full Product Details

Author:   Stephen J. Higgins ,  B. D. Hames
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No. 135
Dimensions:   Width: 15.00cm , Height: 2.30cm , Length: 23.00cm
Weight:   0.610kg
ISBN:  

9780199633449

ISBN 10:   0199633444
Pages:   232
Publication Date:   01 February 1994
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Spiral bound
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Synthesis and purification of RNA substrates; Characterization of RNA; Splicing of mRNA precursors in mammalian cells; Isolation and characterization of ribonucleoprotein complexes; Analysis of ribonucleoprotein interactions; Analysis of pre-mRNA splicing in yeast; Appendix

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