Overview

"presents methods that represent the current ""state-of-the art"" in the fields of RNA interference, editing, and modification; written in such great detail, that prior experience with the technique should not be required to replicate the methods described; by presenting methods developed for a range of organisms, this book lays the ground work for modification or adaptation of assays and approaches for use in other biological systems."

Full Product Details

Author:   Jonatha M. Gott
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   265
Dimensions:   Width: 15.20cm , Height: 2.50cm , Length: 22.90cm
Weight:   1.780kg
ISBN:  

9781588292421

ISBN 10:   1588292428
Pages:   436
Publication Date:   15 April 2004
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

RNA Interference and Gene Silencing.- RNA Interference.- Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans.- Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells.- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference.- Short Hairpin Activated Gene Silencing in Mammalian Cells.- Geminivirus Vectors for Transient Gene Silencing in Plants.- Posttranscriptional Gene Silencing in Plants.- Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning.- RNA Editing and Modification.- A Historical Perspective on RNA Editing.- Identification of Substrates for Adenosine Deaminases That Act on RNA.- Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli.- Isolation of an mRNA-Binding Protein Involved in C-to-U Editing.- In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities.- Identification and Characterization of Trypanosome RNA-Editing Complex Components.- Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro.- Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii.- In Vitro RNA Editing Systems From Higher Plant Chloroplasts.- Studying RNA Editing in Transgenic Chloroplasts of Higher Plants.- Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography.- Functional Characterization of 2?-O-Methylation and Pseudouridylation Guide RNAs.- Experimental Rnomics.

Reviews

...the book delivers detailed experimental protocols that are clearly presented and easy to understand...it delivers practical information.... -ChemBioChem

...the book delivers detailed experimental protocols that are clearly presented and easy to understand...it delivers practical information... -ChemBioChem

.,. the book delivers detailed experimental protocols that are clearly presented and easy to understand...it delivers practical information.... -ChemBioChem

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