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OverviewHigh Quality Content by WIKIPEDIA articles! RiboGreen is a proprietary fluorescent dye that is used in the detection and quantitation of nucleic acids, including both RNA and DNA. It is synthesized and marketed by Molecular Probes/Invitrogen (a division of Life Technologies) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits little fluorescence and possesses a negligible absorbance signature. When bound to nucleic acids, the dye fluoresces with an intensity that, according to the manufacturer, is several orders of magnitude greater than the unbound form. The fluorescence can be detected by a sensor and the nucleic acid can be quantified. The presence of protein contaminants in the sample of nucleic acids to be tested does not make significant contributions to the absorbance, and thus allows for the addition of deoxyribonucleases to the protocol in order to degrade DNA, in the instances where one is only interested in detecting or quantifying RNA. Full Product DetailsAuthor: Lambert M. Surhone , Miriam T. Timpledon , Susan F. MarsekenPublisher: VDM Publishing House Imprint: VDM Publishing House Dimensions: Width: 22.90cm , Height: 0.60cm , Length: 15.20cm Weight: 0.165kg ISBN: 9786131065248ISBN 10: 6131065241 Pages: 104 Publication Date: 28 July 2010 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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