Overview

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Full Product Details

Author:   Valentin Gordeliy
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2501
Weight:   0.714kg
ISBN:  

9781071623312

ISBN 10:   1071623311
Pages:   363
Publication Date:   22 July 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Microbial Rhodopsins.- Molecular Biology of Microbial Rhodopsins.- Rhodopsin-Based Optogenetics: Basics and Applications.- Searching Metagenomes for New Rhodopsins.- E. coli Expression and Purification of Microbial and Viral Rhodopsins.- Crystallization of Microbial Rhodopsins.- Crystallographic Studies of Rhodopsins: Structure and Dynamics.- Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins.- Solid-State NMR Spectroscopy on Microbial Rhodopsins.- FTIR and Raman Spectroscopy of Rhodopsins.- Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins.- Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and ΔpH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches.- Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments.- Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications.- Optogenetic Studies of Mitochondria.- In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins.- Optogenetic Control of Human Stem Cell-Derived Neurons.

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