Overview

Leading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products - recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

Full Product Details

Author:   Rocky S. Tuan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1997 ed.
Volume:   63
Dimensions:   Width: 15.50cm , Height: 3.00cm , Length: 23.50cm
Weight:   0.956kg
ISBN:  

9780896034815

ISBN 10:   089603481
Pages:   448
Publication Date:   05 March 1997
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Overview of Experimental Strategies on the Detection and Isolation of Recombinant Proteins and Their Applications.- Detection and Isolation of Expressed Proteins.- Reporter Systems.- Detection of Recombinant Protein Based on Reporter Enzyme Activity: Chloramphenicol Acetyltransferase.- Human Placental Alkaline Phosphatase as a Marker for Gene Expression.- Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cells.- Detection of ?-Galactosidase and ?-Glucuronidase Using Chemiluminescent Reporter Gene Assays.- Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins.- Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antibody Overlay.- Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporter.- Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein A.- Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteins.- Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins.- Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoprotein.- Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatography: p53.- Yeast GAL4 Two-Hybrid System.- Alternative Yeast Two-Hybrid Systems.- Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokinase-Type Plasminogen Activator.- Detection of Recombinant Gene Expression In Situ.- Histochemical and Fluorochrome-Based Detection of ?-Galactosidase.- Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section.- Whole Mount In SituHybridization to Embryos and Embryonic Tissues.- Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes.- Methods for Double Detection of Gene Expression.- In Situ PCR.- Expression and Detection of Green Fluorescent Protein (GFP).- Applications of Recombinant Gene Expression.- Transgenic Animals as Bioreactors for Expression of Recombinant Proteins.- Artificial Cells for Bioencapsulation of Cells and Genetically Engineered E. coli.- Hyperexpression of a Synthetic Protein-Based Polymer Gene.- Transplantation of Cells in an immunoisolation Device for Gene Therapy.- Delivery of Recombinant HIV-1-Directed Antisense and Ribozyme Genes.- Inducible System Designed for Future Gene Therapy.- Human Gene Therapy.

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