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OverviewThe general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology. Full Product DetailsAuthor: Otto-Wilhelm Merten , D. Mattanovich , C. Lang , G. LarssonPublisher: Springer Imprint: Springer Edition: Softcover reprint of hardcover 1st ed. 2001 Dimensions: Width: 15.50cm , Height: 2.10cm , Length: 23.50cm Weight: 0.635kg ISBN: 9789048157563ISBN 10: 9048157560 Pages: 400 Publication Date: 01 December 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Out of stock  The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available. Table of ContentsCell-free translation systems.- Protein synthesis and co-translational folding in cell-free translation systems.- Metabolic burden and stress response.- The cellular response to unfolded proteins in the endoplasmic reticulum.- The effects of recombinant protein expression on the growth and metabolism of mammalian cells.- Mapping stresses in Escherichia coli to improve yield. Examining global gene regulation and « cell conditioning » strategies.- Cellular responses to strong overexpression of recombinant genes in Escherichia coli. DNA relaxation and cell death after induction of ?-glucosidase.- From Vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteins.- Genetic stability and gene copy number effects.- Protein mass production in hybridomas and recombinant CHO cells.- Inducible gene copy number amplification for the production of heterologous proteins in Kluyveromyces lactis.- Antibiotic-free plasmid selection and maintenance in Bacteria.- Modelling of segregational plasmid instability of recombinant strain suspension of Escherichia coli.- Transcription, translation, and product formation in E. coli.- Production of optically pure aryl epoxides by recombinant E. coli carrying styrene monooxygenase. A new biocatalyst based on Pseudomonas fluorescens ST genes.- Translational problems associated with the rare arginine CGG in Escherichia coli. Frameshifhting at CGG codons.- Optimisation of the solubility of the recombinant Itk kinase domain in Escherichia coli.- Bacterial senescence and the oxidation paradox.- Metabolic approaches for the optimisation of recombinant fermentation processes.- Control and optimisation of cellular bottlenecks in recombinant protein production.- Expression and fermentation strategies for recombinant proteinproduction in Escherichia coli.- Transcription, translation, and product formation in microbial systems other than E. coli.- Overexpression of a Rhizopus oryzae lipase in Pichia pastoris strains containing multiple copies of the target gene.- Development of a heterologous gene expression system for use in Lactococcus lactis. A novel gram-positive expression system.- Metabolic network analysis for human therapeutic protein productions: effects of the P/O ratio.- Animal cell based expression systems — process optimisation.- Process-orientated metabolic engineering: cell lines with new properties in nutrient exploitation and protein glycosylation.- Influence of the metabolic status of packaging cells on retroviral vector production.- Optimizing the production of recombinant prion protein from CHO cells.- Recombinant protein production by transient transfection of suspension-growing cells.- Comparison of different microbial expression systems.- Production of recombinant human trypsinogen in Escherichia coli and Pichia pastoris. A comparison of expression systems.- Secretion of homologous and heterologous recombinant proteins in Escherichia coli and other gram-negative bacteria by using a new secretion system.- Monitoring of genes that respond to overproduction of insoluble recombinant proteins in Escherichia coli and Bacillus subtilis.- Model supported optimization of fed-batch fermentations for recombinant protein production.- Protease secretion capacity and performance analysis of recombinant Bacillus species.- Authors Index.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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