Overview

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Full Product Details

Author:   Charles Z. Hotz ,  Marcel Bruchez
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2007 ed.
Volume:   374
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   0.402kg
ISBN:  

9781627039185

ISBN 10:   162703918
Pages:   257
Publication Date:   14 November 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Imaging of Fixed Cells and Tissues.- Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates.- Immunohistochemical Detection With Quantum Dots.- Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging.- Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots.- Human Metaphase Chromosome FISH Using Quantum Dot Conjugates.- Imaging of Live Cells.- Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors.- Single Quantum Dot Tracking of Membrane Receptors.- Optical Monitoring of Single Cells Using Quantum Dots.- Peptide-Mediated Intracellular Delivery of Quantum Dots.- Multiple Cell Lines Using Quantum Dots.- Measuring Cell Motility Using Quantum Dot Probes.- Imaging of Live Animals.- Quantum Dots for In Vivo Molecular and Cellular Imaging.- Sentinel Lymph Node Mapping With Type-II Quantum Dots.- Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma.- Flow Cytometry Applications.- Application of Quantum Dots to Multicolor Flow Cytometry.- Quantum Dots in Flow Cytometry.- Biochemical Applications.- Luminescent Biocompatible Quantum Dots.- Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots.- Application of Quantum Dots to Multicolor Microarray Experiments.

Reviews

From the reviews: ...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications. -Anticancer Research 27, May 2007 Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ... in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging. (Neel Ghosh, ChemBioChem, Issue 9, 2008)

From the reviews: ...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications. -Anticancer Research 27, May 2007 Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ... in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging. (Neel Ghosh, ChemBioChem, Issue 9, 2008)

From the reviews: .. .provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications. -Anticancer Research 27, May 2007 Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging. (Neel Ghosh, ChemBioChem, Issue 9, 2008)

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