Overview

Pyrosequencing is a DNA sequencing technique that involves sequencing by synthesis, and employs a series of enzymes to detect nucleic acid sequences during the synthesis and enables qualitative analysis of the DNA. A major advantage of this technique is that the labeled primers and nucleotides, as well as gel-electrophoresis is not required. The method requires minimal amounts of samples and gives a high throughput at a lower cost. Besides, the sequence reaction time per base calling is shortened, with higher accuracy, better automation and parallel processing. Pyrosequencing offers a valuable tool for obtaining and analysis of accurate data, identification of new alleles, polymorphic genetic markers, especially Single Nucleotide polymorphisms. The primary purpose of this book is to discuss basics of the Pyrosequencing technology and its key features, including the general sequencing modes, limitations, and future applications.

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9781773611501

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Dr Prerna Pandey, a biotechnologist with several years of wet lab research experience, worked at International Center for Genetic Engineering and Biotechnology, New Delhi. Her field of research in PhD included isolation and molecular characterization of geminiviruses, genome sequencing, gene annotation, and gene silencing using the RNA interference technology. She has also worked at Transasia Biomedicals and Advance Enzyme Technologies as a scientist. Prerna has published papers in peer-reviewed journals, and has submitted a number of annotated Geminiviral genome sequences in the GenBank, including two novel ones. She has also completed her editing and proof-reading courses from Society for the promotion of Editors and Proof-readers, UK and now works as a freelance scientific editor and writer. When Prerna is not busy with her assignments, she loves to explore historical places.

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