Proximity Labeling: Methods and Protocols

Author:   Murat Sunbul ,  Andres Jäschke
Publisher:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2008
ISBN:  

9781493995363


Pages:   231
Publication Date:   25 May 2019
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Proximity Labeling: Methods and Protocols


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Author:   Murat Sunbul ,  Andres Jäschke
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2008
Weight:   0.695kg
ISBN:  

9781493995363


ISBN 10:   1493995367
Pages:   231
Publication Date:   25 May 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The EMARS Reaction for Proximity Labeling.- Proteomic Proximity Labelling to Reveal Interactions Between Biomolecules.- Establishing Cell Culture-based Experimental Setups for Proximity Labeling Using Ascorbate Peroxidase (APEX).- APEX Peroxidase Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.- Identification of Lipid Droplet Proteomes by Proximity Labeling Proteomics Using APEX2.- Antibody-driven Proximity Labeling in Fixed Tissues.-  BioID: A Method to Generate a History of Protein Associations.- Direct Identification of Biotinylated Proteins from Proximity Labeling (Spot-BioID).-  Identifying Synaptic Proteins by in vivo BioID from Mouse Brain.- A Proximity Ligation-based Method to Detect RNA-DNA Association.- Enzymatic Transfer of Photo-cross-linkers for RNA-Protein Photo-cross-linking at the mRNA 5'-cap.- Proximity-induced Bioorthogonal Chemistry Using Inverse Electron Demand Diels-Alder Reaction.- Overcoming the Limitations of Sortase with Proximity-based Sortase-mediated Ligation (PBSL).- Proximity-driven Site-specific and Covalent Labeling of Proteins with a TexasRed Fluorophore Reacting (ReacTR) Peptide Tag.- Affinity Conjugation for Rapid and Covalent Labeling of Proteins in Live Cells.- Ligand-directed N-Sulfonyl Pyridone Chemistry for Selective Native Protein Labeling and Imaging in Live Cell.

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