Overview

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

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Author:   Shweta Pandey (Assistant Professor, Department of Biotechnology, Govt VYT PG Autonomous College, Durg Chhatisgarh, India) ,  Shyamal K Goswami (Professor (retired), School of Life Sciences, Jawaharlal Nehru University, New Delhi, India) ,  Buddhi Prakash Jain (Assistant Professor, Department of Zoology, School of Life Sciences, Mahatma Gandhi Central University Bihar, Motihari, India) ,  Shyamal K. Goswami (Professor, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.290kg
ISBN:  

9780128220078

ISBN 10:   0128220074
Pages:   138
Publication Date:   16 November 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9780443139451
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Unit I: Buffer, solution and pH 1. Preparation of normal and molar solution 2. Buffer preparation 3. Determination of pKa, pH Unit II: Nucleic Acids 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Purification of Nucleic Acid 4. Quantitative estimation of Nucleic acid 5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids 1. Qualitative test for carbohydrate (Benedict, Molisch etc) 2. Qualitative test for Lipids 3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins 1. Isolation of Protein 2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc) 3. Estimation of Protein (Bradford, Lowry etc) 4. Paper Chromatography for separation and identification of amino acid 5. Ion exchange chromatography for separation of amino acid 6. Protein purification 7. SDS PAGE analysis of proteins 8. Western blotting for protein analysis 9. Isoelectric pH determination Unit IV: Enzymes 1. Enzyme kinetics 2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity 3. Enzymatic activity of Trypsin and Lipase 4. Enzyme purification 5. Determination of Km, Vmax and Ki Unit V: Clinical test 1. Acid and Alkaline phosphatase activity 2. SGPT and SGOT test 3. Estimation of Urea, Uric acid and Creatinine 4. Estimation of Glucose 5. Estimation of Cholesterol 6. Estimation of Vitamins 7. Glucose tolerance test 8. Estimation of Ca2+ and T4 in serum 9. Pregnancy test by HCG hormone 10. Estimation of Glycosylated haemoglobin

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Author Information

Shweta Pandey is an Assistant Professor at the Department of Biotechnology, Govt VYT PG Autonomous College, Durg Chhatisgarh, India. Dr. Pandey received a PhD from the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. She has vast teaching experience and has published several papers in reputed international journals, many book chapters in edited books, and co-edited Protocols in Biochemistry and Clinical Biochemistry (Elsevier, 2020). Her research interests include cell cycle regulation, molecular and cell mechanisms of tumor cells, and cancer biology. Shyamal Goswami has a research career spanning 35 years. He has experience teaching in biochemistry, cellular and molecular biology and redox biology at the postgraduate level. He served as Associate Professor and Professor in the School of Life Sciences at Jawaharlal Nehru University, India, from 1998 to 2020. Prior to this he was a Research Assistant Professor in the Department of Anatomy and Cell Biology at the State University of New York-Health Science Centre at Brooklyn, New York, USA. Dr. Goswami has contributed numerous publications across his field and has more than 1500 citations. He also co-authored Protocols in Biochemistry and Clinical Biochemistry and co-edited Post-transcriptional Gene Regulation in Human Disease, both published by Academic Press/Elsevier. His main areas of research are cell signaling and gene expression in the cardiovascular system; redox biology of degenerative diseases; and signal scaffolds and cell functions. Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.

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