Overview

This text outlines the basic principles and instrumentations defining the new emerging field of nanocrystallography at the frontiers of nanotechnology and proteomics, leading to a transition from art to science and technology in protein crystallography. Dedicated thin biofilm technology, synchrotron radiation and atomic force microscopy along with traditional methods of classical crystallography and of recombinant DNA are described in details.

Full Product Details

Author:   Eugenia Pechkova ,  C. Nicolini
Publisher:   Springer Science+Business Media
Imprint:   Kluwer Academic/Plenum Publishers
Edition:   2003 ed.
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.519kg
ISBN:  

9780306479021

ISBN 10:   0306479028
Pages:   190
Publication Date:   30 September 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1 The New Frontier at the Crossing of life and Physical Sciences.- 2 State of the Art in Proteomics, Crystallography and Nanobiotechnology.- 2.1 Methods of Protein Production.- 2.2 Methods for Protein Identification.- 2.3 Methods of Protein Crystallization.- 2.4 Methods of Crystal Structure Determination.- 2.5 Other Methods of Protein Structure Investigation.- 2.6 Methods of Protein Immobilization In Solid Thin Films.- 3 Nanocrystallography.- 3.1 Nanobiofilms Template.- 3.2 Atomic Force Microscopy.- 3.3 Synchrotron Radiation.- 3.4 From Art to Science With Protein Nanocrystallography.- 4 From Art to Science in Protein Crystallography.- 4.1 New Method Validation With Lysozyme as a Model Protein.- 4.2 Yet Unresolved Proteins: Bovine Cytochrome P450scc.- 4.3 Yet unresolved proteins: Human kinase CK2? Catalytic Subunit.- 4.4 Conclusions.- 5 From Science Technology in Proteomics.- 5.1 Automated Mass Spectrometry.- 5.2 Biomolecular Arrays.- 5.3 Unsolved Protein Structures of Scientific and Industrial Interest.- 6 Future Trends.- 6.1 Novel Composite Biomaterials and Nanodevices.- 6.2 nanocrystals.- References.

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