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OverviewLike the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The topics covered are: nomenclature and classification; purification; assay methods; determination of mechanism; inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing; limited proteolysis as a structural probe; synthetic function. This book will be as invaluable as the first edition in providing ideas and protocols for scientists either studying proteases or using proteases as a research tool. Full Product DetailsAuthor: Rob Beynon (Department of Veterinary Preclinical Studies, Department of Veterinary Preclinical Studies, University of Liverpool) , J. S. Bond (Department of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, The Pennsylvania State University)Publisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: 247 Dimensions: Width: 18.80cm , Height: 2.00cm , Length: 24.50cm Weight: 0.775kg ISBN: 9780199636624ISBN 10: 0199636621 Pages: 358 Publication Date: 11 January 2001 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: To order  Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of ContentsProteolytic Enzymes: nomenclature and classification Purification of Proteolytic Enzymes Protease Assay Methods Determination of Protease Mechanism Inhibition of Proteolytic Enzymes Finding, Purification and Characterization of Natural Protease Inhibitors Mass Spectrometry of Proteolysis-derived Peptides for Protein Identification Using Proteinases for Edman Sequence Analysis and Peptide Mapping Prevention of Unwanted Proteolysis Proteolysis of Native Proteins as a Structural Probe Proteases in Peptide SynthesisReviewsProteolytic enzymes lives up to its subtitle a practical approach. It provides a background to newcomers and students in the field, and will serve as a reference book for anyone working in this area. --Biochemistry and Molecular Biology Education<br> Proteolytic enzymes lives up to its subtitle a practical approach. It provides a background to newcomers and students in the field, and will serve as a reference book for anyone working in this area. --Biochemistry and Molecular Biology Education <br> Proteolytic enzymes lives up to its subtitle a practical approach. It provides a background to newcomers and students in the field, and will serve as a reference book for anyone working in this area. --Biochemistry and Molecular Biology Education<p><br> Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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