Proteoglycans: Methods and Protocols

Author:   Nikos K. Karamanos
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2619
ISBN:  

9781071629451


Pages:   317
Publication Date:   21 January 2023
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Proteoglycans: Methods and Protocols


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Author:   Nikos K. Karamanos
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2619
Weight:   0.823kg
ISBN:  

9781071629451


ISBN 10:   107162945
Pages:   317
Publication Date:   21 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Glycosaminoglycans: Strategies for Detection and Structure Characterization1. Detection of Glycosaminoglycans in Biological Specimens Shaukat A Kha, FNU Nidhi, Paige C. Amendu, and Shunji Tomatsu 2. Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization Of Chondroitin Sulfate ProteoglycansTadahisa Mikami and Hiroshi Kitagawa 3. Proteoglycans: A Tool for detecting hyaluronan by ELISA-like MethodsDaiana L. Vitale, Rosalía I. Cordo-Russo, and Laura Alaniz 4. Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular MatrixArianna Parnigoni, Paola Moretto, Simona Rovera, Manuela Viola, Evgenia Karousou, Alberto Passi, and Davide Vigetti 5. Urinary Glycosaminoglycans: Characterization and QuantificationNaima Fdil and Es-Said Sabir 6. HPLC-MS characterization of tissue-derived heparan sulfate and chondroitin sulfateGábor Tóth, Lilla Turiák 7. Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues Chrysostomi Gialeli and Isabel Gonçalves 8. Studying the effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron MicroscopyChristos Koutsakis, Marco Franchi, Anastasia-Gerasimoula Tavianatou, Valentina Masola, and Nikos K. Karamanos Part II: Proteoglycans: Separation, Analysis, and Interactions9. Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate ImmunityJinyang Zeng-Brouwers, Lisa Sophie Huber, Rosetta Merline, Jonel Trebicka, Malgorzata Wygrecka, and Liliana Schaefer 10. Analysis of Extracellular Vesicle-associated ProteoglycansJuliana Poças and Mattias Belting 11. Analysis of Aggrecan Glycanation by Western Blot in Cell CultureChiara Gramegna Tota, Alessandra Leone, Antonio Rossi, and Chiara Paganini 12. Repulsive Scaling Replica Exchange Molecular Dynamics in Modeling Protein-Glycosaminoglycan ComplexesMateusz Marcisz, Martyna Maszota-Zieleniak, and Sergey A. Samsonov 13. In vitro investigation of hyaluronan/CD44 networkTheodore Karalis, Paraskevi Heldin, and Spyridon S. Skandalis 14. Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and ImmunoblottingJames Melrose 15. Building, Visualizing, and Analyzing Glycosaminoglycan-Protein Interaction NetworksSylvie Ricard-Blum Part III: Proteoglycans related Molecules (Enzymes, Inhibitors, and Epigenetics)16. An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying KineticsEdward Hammond and Vito Ferro 17. A method for Urinary Trypsin Inhibitor (UTI) purification combining anion-exchange chromatography enrichment and preparative SDS-PAGEGabriele Nieddu, Marilena Formato, and Antonio Junior Lepedda 18. Enzymatic Sequencing of Heparin Oligosaccharides Using ExolyaseQingdong Zhang, Danrong Lu, and Fuchuan Li 19. Evaluating the Effects of microRNAs on Proteoglycans and Matrix Constituents’ Expression and Functional PropertiesZoi Piperigkou and Nikos K. Karamanos  20. Proteoglycan Expression Studied by microRNAsNancy Adriana Espinoza-Sanchez, Fabian Troschel, Burkhard Greve, and Martin Götte 21. Modeling and simulations of Extra Cellular GlycoproteinsRajas M Rao, Nicolas Belloy, Jean-Marc Crowet, Manuel Dauchez, and Stéphanie Baud

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