Overview

Expert researchers describe the key methods for detecting and analyzing proteins and protein motifs involved in targeting interactions, mostly from the perspective of intracellular signaling. The techniques include those designed to reveal the existence of targeting interactions and characterize their physico-chemical properties, to identify and isolate interacting proteins on the basis of the bait-prey' principle, to target domains, and to modify lipids. The methods are richly detailed for use by those researchers interested in defining and characterizing the protein protein interactions that underlie targeting mechanisms in all areas of molecular cell biology.

Full Product Details

Author:   Roger A. Clegg
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   88
Dimensions:   Width: 15.20cm , Height: 1.90cm , Length: 22.90cm
Weight:   1.130kg
ISBN:  

9780896034501

ISBN 10:   089603450
Pages:   331
Publication Date:   14 January 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9781588297020
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Surface Plasmon Resonance.- Microcalorimetry of Protein-Protein Interactions.- Partitioning of Proteins in Triton X-114.- Co-Immunoprecipitation.- Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring.- Size-Exclusion Chromatography.- Overlay and Bead Assay.- Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins.- Molecular Genetic Approaches I.- Molecular Genetic Approaches II.- Molecular Genetic Approaches III.- Molecular Genetic Approaches IV.- Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions.- SH2 and SH3 Domains.- PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB).- Membrane Targeting via Protein N-Myristoylation.- Membrane Targeting via Protein Palmitoylation.- Agonist-Mediated Turnover of G-Protein ?-Subunit Palmitoyl Groups.- Membrane Targeting via Protein Prenylation.- Nuclear Protein Import in a Permeabilized Cell Assay.- Clathrin-Coated Vesicles and Targeting.- Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway.

Reviews

"""... a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers...""-The Quarterly Review of Biology"

a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers - The Quarterly Review of Biology

... a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers... -The Quarterly Review of Biology

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