Overview

This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.

Full Product Details

Author:   Robin Martin
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   77
Dimensions:   Width: 15.50cm , Height: 3.00cm , Length: 23.50cm
Weight:   1.860kg
ISBN:  

9780896033979

ISBN 10:   089603397
Pages:   442
Publication Date:   04 August 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Analyses of Ribosome Distribution During In Vitro Translation.- Allele-Specific Priming in the Mapping of rRNA.- Permeabilized Mammalian Cells as a System for Protein Synthesis.- The Measurement of Processivity Errors in Protein Synthesis.- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins.- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A.- In Vitro Engineering Using Acyl-Derivatized tRNAs.- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons.- Analysis of rRNA Function Using Specialized Ribosomes.- Determination of the Peptide Elongation Rate In Vivo.- The Measurement of Missense Errors During In Vivo Protein Synthesis.- Heelprinting Analysis of In Vivo Ribosome Pause Sites.- Analysis of elF-2? Kinases in Yeast.- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems.- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis.- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors.- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems.- Measurement of Rate of Protein Synthesis In Vitro.- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae.- Genetic Selection of rRNA Mutations.- Toeprinting Assays.- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution.- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes.- Protein Engineering with Nonstandard Amino Acids.- Internal Ribosome Entry Sites Tests with Circular mRNAs.- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or3? UTRs of Translationally Regulated mRNAs.- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs.- Identification and Analysis of Frameshift Sites.- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.

Reviews

... an excellent protocols book, a copy of which should be on the shelves of every molecular biology lab. - Microbiology Today

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