Overview

This new edition of Protein Purification Protocols (1996) completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.

Full Product Details

Author:   Paul Cutler
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2004
Volume:   244
Dimensions:   Width: 17.80cm , Height: 2.60cm , Length: 25.40cm
Weight:   1.128kg
ISBN:  

9781588290670

ISBN 10:   1588290670
Pages:   484
Publication Date:   12 December 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

General Strategies.- Preparation of Extracts From Animal Tissues.- Protein Extraction From Plant Tissues.- Extraction of Recombinant Protein From Bacteria.- Protein Extraction From Fungi.- Subcellular Fractionation of Animal Tissues.- Subcellular Fractionation of Plant Tissues.- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds.- Avoidance of Proteolysis in Extracts.- Concentration of Extracts.- Making and Changing Buffers.- Purification and Concentration by Ultrafiltration.- Bulk Purification by Fractional precipitation.- Ion-Exchange Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- Lectin Affinity Chromatography.- Immunoaffinity Chromatography.- Immobilized Metal-Ion Affinity Chromatography.- Chromatography on Hydroxyapatite.- Thiophilic Affinity Chromatography and Related Methods.- Affinity Precipitation Methods.- Isoelectric Focusing.- Chromatofocusing.- Size-Exclusion Chromatography.- Fast Protein Liquid Chromatography.- Reversed-Phase Chromatography of Proteins.- Extraction of Membrane Proteins.- Removal of Detergent From Protein Fractions.- Purification of Membrane Proteins.- Lyophilization of Proteins.- Storage of Pure Proteins.- Electroelution of Proteins From Polyacrylamide Gels.- Electroblotting of Proteins From Polyacrylamide Gels.- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses.- Microscale Solution Isoelectrofocusing.- Practical Column Chromatography.- Detection Methods.- Peptide Proteomics.- Multidimensional Liquid Chromatography of Proteins.- Mass Spectrometry.- Purification of Therapeutic Proteins.- Purification Process Scale-Up.

Reviews

From reviews of the first edition very useful... - Angewandte Chemie: International Edition valuable to keep around the laboratory - Biopharm recommended for protein chemists. There is something in this book for everyone. an excellent working book as well as an excellent reference. - JAOCS: Journal of the American Oil Chemists' Society an excellent lab companion - SIM News a worthwhile purchase for novice and established practitioners alike. - Microbiology Today ...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists. - Biomedical Chromatography

From reviews of the first edition... ...very useful... -Angewandte Chemie: International Edition ...valuable to keep around the laboratory... -Biopharm ...recommended for protein chemists...There is something in this book for everyone...an excellent working book as well as an excellent reference. -JAOCS: Journal of the American Oil Chemists' Society ...an excellent lab companion... -SIM News ...a worthwhile purchase for novice and established practitioners alike. -Microbiology Today ...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists. -Biomedical Chromatography Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners. -SGM Quarterly

From reviews of the first edition... ...very useful... -Angewandte Chemie: International Edition ...valuable to keep around the laboratory... -Biopharm ...recommended for protein chemists....There is something in this book for everyone....an excellent working book as well as an excellent reference. -JAOCS: Journal of the American Oil Chemists' Society ...an excellent lab companion... -SIM News ...a worthwhile purchase for novice and established practitioners alike. -Microbiology Today ...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists. -Biomedical Chromatography Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners. -SGM Quarterly

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