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Author:   Philip Bonner
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Edition:   2nd edition
Weight:   0.516kg
ISBN:  

9780815344889

ISBN 10:   0815344880
Pages:   269
Publication Date:   16 August 2018
Audience:   College/higher education ,  Professional and scholarly ,  Tertiary & Higher Education ,  Professional & Vocational
Replaced By:   9780367223663
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Water, pH and buffers. 2. Protein structure and properties. 3. Chromatography and the strategy of protein purification. 4. Groundwork. 5. Non-affinity absorption techniques used to purify proteins. 6. Affinity-based procedures used to purify proteins. 7. Non-absorption techniques for purifying proteins. 8. Methods for monitoring the purity of protein solutions. Appendix 1: The laws of thermodynamics and the Gibbs free energy equation. Appendix 2: Properties of amino acids. Appendix 3: The common units used in biology. Appendix 4: Chromatographic runs. Appendix 5: Determining the concentration of a compound in a solution in parts per million (ppm). Appendix 6: The answers to the exercises in the various chapters. Appendix 7: An alphabetical list of chromatography, electrophoresis, filtration equipment and laboratory suppliers. Appendix 8: Buffer tables to prepare at a required pH value.

Reviews

. ..books such as this are a useful reference source. - BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, Vol. 36, 2008

Author Information

Dr Philip Bonner is Programme Leader for the MRes in Applied Biosciences at Nottingham Trent University.

Tab Content 6

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