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OverviewFull Product DetailsAuthor: Cheryl L. Meyerkord , Haian FuPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of the original 2nd ed. 2015 Volume: 1278 Weight: 1.613kg ISBN: 9781493949854ISBN 10: 1493949853 Pages: 620 Publication Date: 29 October 2016 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsStructural basis of protein-protein interactions.- Quantitative analysis of protein-protein interactions.- Protein-protein interaction databases.- Computational prediction of protein-protein interactions.-- Structure-based computational approaches for small-molecule modulation of protein-protein interactions.- Targeting protein-protein interactions for drug discovery.- Studying protein-protein interactions using surface plasmon resonance.- Resonant waveguide grating for monitoring bimolecular interactions.- Quartz microbalance technology for probing biomolecular interactions.- Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry.- Characterization of protein-protein interactions by isothermal titration calorimetry.- Sedimentation equilibrium studies.- Detecting protein-protein interactions by gel filtration chromatography.- Using light scattering to determine the stoichiometry of protein complexes.- Circular dichroism (CD) analyses of protein-protein interactions.- Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy.- Quantitative protein analysis by mass spectrometry.- Using peptide arrays created by the SPOT method for defining protein-protein interactions.- Fluorescence polarization assay to quantify protein-protein interactions.- Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions.- Utilizing ELISA to monitor protein-protein interactions.- Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions.- Hexahistidine (6xHis) fusion-based assays for protein-protein interactions.- Studying protein-protein interactions via blot overlay/Far Western blot.- Co-immunoprecipitation from transfected cells.- In vivo protein cross-linking.- Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening.- Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae .-MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions.- Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells.- Mapping biochemical networks with protein fragment complementation assays.- Detection of protein-protein interaction using bimolecular fluorescence complementation assay.- Split-luciferase complementation assay to detect channel-protein interactions in live cells.- Confocal microscopy for intracellular co-localization of proteins.- Fluorescence polarization assay to quantify protein-protein interactions in an HTS format.- Estrogen receptor alpha/co-activator interaction assay - TR-FRET.- High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions.- Discovery of inhibitors of the MDM2-p53 protein-protein interaction.- Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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