Overview

Written by an experienced and well-published individual, this unique reference source takes a forward-looking approach. It describes the concepts and practice of protein phosphorylation analysis by tandem mass spectrometry and related techniques. These include purification, enrichment, database searching, other software tools, synthesis, phosphatase treatment, phospho-specific staining methods, isoelectric focusing and element mass spectrometry. The book then goes on to cover the fragmentation behaviour of phosphopeptides in tandem MS (pos+neg ions) and the implementation of the particular features into an analytical strategy. The book ends with a summary and discussion of useful internet and software tools currently available.

Full Product Details

Author:   Wolf D Lehmann (German Cancer Research Center, Germany)
Publisher:   Royal Society of Chemistry
Imprint:   Royal Society of Chemistry
Dimensions:   Width: 15.60cm , Height: 2.20cm , Length: 23.40cm
Weight:   1.604kg
ISBN:  

9780854041855

ISBN 10:   0854041850
Pages:   396
Publication Date:   01 November 2010
Audience:   College/higher education ,  Professional and scholarly ,  Tertiary & Higher Education ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Reviews

The book is a great and truly insightful guide to understand basics of mass spectrometry and its analytical power for protein phosphorylation analysis. Mogjib Salek Overall, this book positively stands out from the crowd of publications related to biochemical applications of MS.The book clearly excels when concepts, strategies, and applications are discussed,The book can be fully recommended to any practitioner in the field of protein analysisa valuable package for every proteomics laboratory.Overall, the book is a welcome addition to the field of mass-spectrometric protein analysis.it was carefully prepared by the publisher, and yet, it is compact and handy. Jurgen H Gross

The book is a great and truly insightful guide to understand basics of mass spectrometry and its analytical power for protein phosphorylation analysis. * Mogjib Salek * Overall, this book positively stands out from the crowd of publications related to biochemical applications of MS.The book clearly excels when concepts, strategies, and applications are discussed,The book can be fully recommended to any practitioner in the field of protein analysisa valuable package for every proteomics laboratory.Overall, the book is a welcome addition to the field of mass-spectrometric protein analysis.it was carefully prepared by the publisher, and yet, it is compact and handy. * Jurgen H Gross *

Author Information

Wolf D. Lehmann studied at the Universities of Bonn and Z?rich before gaining his PhD in 1975. He is currently based at the German Cancer Research Center in Heidelberg, Germany. His areas of expertise include analytical biochemistry, liquid chromatography, mass spectrometry, protein analysis, covalent protein modifications, and peptide sequencing by tandem mass spectrometry. He has published 120 peer-reviewed articles, made numerous book contributions and is on the Advisory Board of the FEBS Journal. He is also a member of the Society of German Chemists, German Society for Mass Spectrometry and American Society for Mass Spectrometry.

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