Overview

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.

Full Product Details

Author:   Grahame Hardie (Department of Biochemistry, University of Dundee)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   211
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.40cm
Weight:   0.709kg
ISBN:  

9780199637287

ISBN 10:   0199637288
Pages:   460
Publication Date:   14 October 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Preface 1: Haystead and Garrison: Study of protein phosphorylation in intact cells 2: Naito, Kobayashi and Hidaka: Analysis of signal transduction pathways using protein kinase inhibitors and activators 3: Hardie: Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors 4: Sutherland: Analysis of signal transduction pathways using molecular biological manipulations 5: Van der Geer et al: Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates 6: Mitchellhill and Kemp: Phosphorylation site analysis by mass spectrometry 7: Mackintosh and Moorhead: Assay and purification of protein (serine/threonine) phosphatases 8: Tonks: [chapter title missing] 9: Garton, Flint and Tonks: Identification of substrates for protein-tyrosine phosphatases 10: Hardie et al: Assay and purification of protein-serine/threonine kinases 11: Ruzzene and Pinna: Assay of protein kinases and phosphatases using specific peptide substrates 12: Carling: Cloning and expression of cDNAs encoding protein kinase subunits 13: Stone & Walker: Interaction cloning of protein kinase partners 14: Fukunaga and Hunter: Identifying protein kinase substrates by expression screening with solid-phase phosphorylation 15: Jiang & Carlson: Analysis of protein kinase interactions using the two-hybrid method 16: Herberg & Zimmerman: Analysis of protein kinase interactions using biomolecular interaction analysis 17: Songyang and Cantley: Analysis of protein kinase specificity using orientated peptide libraries 18: Kobe et al: Crystallization of protein kinases and phosphatases Index

Reviews

This will be an invaluable laboratory aid to researches involved in this field Aslib Book Guide

"""This will be an invaluable laboratory aid to researches involved in this field"" Aslib Book Guide"

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